Antidiarrhea Effect Test Ethanol Extract of Green Grass Jelly Leaves with a Defecation Method in Mice

Oct 13, 2025 by thesweetdesigns.com 109 views

Antidiarrhea Effect Test Ethanol Extract of Green Grass Jelly Leaves with a Defecation Method in Mice

Introduction

Green grass jelly, a plant known by the people of Indonesia, especially in Java, has been used for centuries as a traditional food and treatment for various complaints such as heat, stomach ache, and diarrhea. This plant is also known to contain antioxidant compounds that have the potential to have anticancer activity. The aim of this study is to examine the characteristics of the simplicia of green grass jelly leaves and the antidiarrheal effect of the ethanol extract of green grass jelly leaves in mice induced by oleum ricini.

Background of Green Grass Jelly

Green grass jelly is a plant that has been used for centuries in Indonesia, especially in Java. It is not only used as a traditional food but also as a treatment for various complaints. The plant is known to contain antioxidant compounds that have the potential to have anticancer activity. The use of green grass jelly as a treatment for various complaints is a testament to its potential as a medicinal plant. However, there is limited research on the antidiarrheal effect of green grass jelly leaves.

Methodology

In this study, the simplicia powder of green grass jelly leaves was characterized and carried out phytochemical screening. The leaf powder is then extracted using 96% ethanol with the maceration method. To test the antidiarrheal effect, the ethanol extract of green grass jelly leaves is given orally to mice at a dose of 50, 100, and 150 mg/kg body weight. As a comparison, HCl's loperramid is given at a dose of 1 mg/kg body weight. Observations were made every 30 minutes for 6 hours related to diarrhea, including when it began, stool consistency, frequency, and duration of diarrhea.

Characterization of Simplicia Green Grass Jelly Leaves

The results of the characterization of simplicia green grass jelly leaves show that the water content reaches 5.2%, water soluble juice content is 20.63%, ethanol soluble juice levels 17.24%, total ash content of 14.28%, and ash content is not acid soluble 0.54%. These results indicate that the simplicia of green grass jelly leaves contains a high amount of water and ethanol soluble compounds.

Phytochemical Screening

The results of phytochemical screening show the presence of alkaloidal compounds, flavonoids, glycosides, tannins, and steroids/triterpenoids. These compounds are known to have various biological activities, including antioxidant, anti-inflammatory, and antimicrobial activities. The presence of these compounds in green grass jelly leaves suggests that it has potential as a medicinal plant.

Antidiarrheal Effect Test

The results of the antidiare effect test show that all doses of green grass jelly leaf extract have a significant antidiarrheal effect. Dose of 50 mg/kg body weight shows a smaller effect compared to Loperramid HCl dose of 1 mg/kg body weight (p <0.05), while a dose of 100 mg/kg body weight has an antidiarrheal effect equivalent to HCl liperramid 1 mg/kg body weight. The dose of 150 mg/kg body weight prolongs the time to start diarrhea and shorten the duration of diarrhea compared to HCl loperramid.

Statistical Analysis

Through statistical analysis, found a significant difference between the dose of 150 mg/kg body weight from green grass jelly leaf extract and 1 mg/kg body weight (P> 0.05). This shows that the ethanol extract of green grass jelly leaves has the potential as an antidiarrheal drug, so that it can be a safe and effective traditional treatment.

Conclusion

This research provides important information about the antidiarrheal effects of green grass jelly leaves. The results of this study suggest that the ethanol extract of green grass jelly leaves has potential as an antidiarrheal drug. The development of herbal medicine in Indonesia is an important aspect of cultural heritage and public health. This research can make a valuable contribution to the development of science in the field of pharmacology, especially related to local medicinal plants that have great potential to be used as an alternative treatment.

Recommendations

Based on the results of this study, it is recommended that further research be conducted to investigate the antidiarrheal effect of green grass jelly leaves in humans. Additionally, the development of herbal medicine in Indonesia should be continued and supported. The use of local medicinal plants as alternative treatments can provide a safe and effective way to manage various complaints.

Limitations

This study has several limitations. The first limitation is that the study was conducted in mice, which may not be representative of humans. The second limitation is that the study only investigated the antidiarrheal effect of green grass jelly leaves, and not other potential biological activities. Further research is needed to investigate the potential biological activities of green grass jelly leaves.

Future Directions

This study provides a foundation for further research on the antidiarrheal effect of green grass jelly leaves. Future studies should investigate the potential biological activities of green grass jelly leaves, including antioxidant, anti-inflammatory, and antimicrobial activities. The development of herbal medicine in Indonesia should be continued and supported.
Q&A: Antidiarrhea Effect Test Ethanol Extract of Green Grass Jelly Leaves with a Defecation Method in Mice

Q: What is green grass jelly?

A: Green grass jelly is a plant that has been used for centuries in Indonesia, especially in Java. It is not only used as a traditional food but also as a treatment for various complaints.

Q: What is the aim of this study?

A: The aim of this study is to examine the characteristics of the simplicia of green grass jelly leaves and the antidiarrheal effect of the ethanol extract of green grass jelly leaves in mice induced by oleum ricini.

Q: What is the method used in this study?

A: In this study, the simplicia powder of green grass jelly leaves was characterized and carried out phytochemical screening. The leaf powder is then extracted using 96% ethanol with the maceration method. To test the antidiarrheal effect, the ethanol extract of green grass jelly leaves is given orally to mice at a dose of 50, 100, and 150 mg/kg body weight.

Q: What are the results of the characterization of simplicia green grass jelly leaves?

A: The results of the characterization of simplicia green grass jelly leaves show that the water content reaches 5.2%, water soluble juice content is 20.63%, ethanol soluble juice levels 17.24%, total ash content of 14.28%, and ash content is not acid soluble 0.54%.

Q: What are the results of phytochemical screening?

A: The results of phytochemical screening show the presence of alkaloidal compounds, flavonoids, glycosides, tannins, and steroids/triterpenoids.

Q: What are the results of the antidiare effect test?

A: The results of the antidiare effect test show that all doses of green grass jelly leaf extract have a significant antidiarrheal effect. Dose of 50 mg/kg body weight shows a smaller effect compared to Loperramid HCl dose of 1 mg/kg body weight (p <0.05), while a dose of 100 mg/kg body weight has an antidiarrheal effect equivalent to HCl liperramid 1 mg/kg body weight. The dose of 150 mg/kg body weight prolongs the time to start diarrhea and shorten the duration of diarrhea compared to HCl loperramid.

Q: What is the significance of this study?

A: This study provides important information about the antidiarrheal effects of green grass jelly leaves. The results of this study suggest that the ethanol extract of green grass jelly leaves has potential as an antidiarrheal drug.

Q: What are the limitations of this study?

A: This study has several limitations. The first limitation is that the study was conducted in mice, which may not be representative of humans. The second limitation is that the study only investigated the antidiarrheal effect of green grass jelly leaves, and not other potential biological activities.

Q: What are the future directions of this study?

A: This study provides a foundation for further research on the antidiarrheal effect of green grass jelly leaves. Future studies should investigate the potential biological activities of green grass jelly leaves, including antioxidant, anti-inflammatory, and antimicrobial activities.

Q: What is the potential of green grass jelly leaves as a medicinal plant?

A: The results of this study suggest that green grass jelly leaves have potential as a medicinal plant. The presence of various bioactive compounds in green grass jelly leaves suggests that it may have various biological activities, including antioxidant, anti-inflammatory, and antimicrobial activities.

Q: What are the implications of this study for the development of herbal medicine in Indonesia?

A: This study highlights the importance of the development of herbal medicine in Indonesia. The use of local medicinal plants as alternative treatments can provide a safe and effective way to manage various complaints.